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Leptin Leptin is a 16 kDa, 146 amino acid residue
non-glycosylated protein hormone involved in regulating body weight,
metabolism and reproductive function. It is encoded by the obese (ob) gene
and expressed predominantly by adipocytes consistent with the fact that body
weight is sensed mainly as the total mass of fat in the body. Leptin is also
secreted by cells in the epithelium of the stomach and in the placenta in
smaller amounts. Leptin receptors are highly expressed in areas of the
hypothalamus that are known to be important in regulating body weight, as
well as in T lymphocytes and vascular endothelial cells.
Catalog Number Size Price L1102S-Human 2.0mg $298.00 L1102L-Human 5.0mg $448.00 L1103S-Mouse 1.0mg $148.00 L1103L-Mouse 10.0mg $898.00 *Please call for quote
if larger sizes needed. Formulation:
Powder
lyophilized from volatile buffer
50mM NH4HCO3 (pH 8.0). Preservative: None. MW: 16kDa Purity: >97% (15%
SDS-PAGE) Source: Recombinant
protein expressed and purified from E.
coli. Sterility: 0.2
µm membrane-filtered
and packaged aseptically. ED50: ND Endotoxin: ≤0.1 EU/mg (determined by Limulus Amebocyte
Lysate (LAL) Assay
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Reconstitution and Use: Reconstitute
the contents of the vial using a sterile buffer (pH8.0 or above) to a concentration
no less than 100 mg/ml and aliquot for future use. (If the initial rehydration is too
dilute, activity may be lost due to the non-specific adsorption to the
container). The solution can then be further diluted to a working stock
solution. If the product is
going to be used for applications requiring absolute asepsis, it’s best to
filter-sterilize the solution using a sterile and non-pyrogenic 0.2 µm membrane before use. Storage
and Stability: Upon receiving, store the product at -20°C.
After reconstitution, store the working aliquots at 2-8 °C for no more than 3 months. For
extended storage, aliquot the rehydrated solution (³100mg/ml) and freeze at -70°C
or -20°C. Avoid
repeated freezing and thawing. Dilute solutions stored at -20°C could lose activity faster. Further
Information: Cherhab FF, Mounzih K, Lu R,
Lim ME: Early onset of reproductive function in normal female mice treated
with leptin. Science 275:88, 1997. Clement K, Vaisse C, Lahlou N,
et al: A mutation in the human leptin receptor gene causes obesity and
pituitary dysfunction. Nature 392:398, 1998. Considine RV, Sinha MK, Heiman
ML etc: Serum immunoreactive-leptin concentrations in normal-weight and obese
humans. New Eng J Med 334:292, 1996. Friedman JM, Halaas JL: Leptin
and the regulation of body weight in mammals. Nature 395:763, 1998. Halaas JL, Pelleymounter MA, Cullen MJ,
Baker MB, etc: Effects of the obese gene product on body weight regulation in
ob/ob mice. Science 269:540, 1995. Zhang Y, Proenca R, Maffei M,
etc: Positional cloning of the mouse obese gene and its human homologue.
Nature 372:425, 1994.). |
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ã2004 Biomyx
Technology · |
