Leptin, Recombinant leptin, mouse leptin, recombinant mouse leptin, human leptin, recombinant human leptin, obesity gene, obese gene

mouse/human Leptin

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Leptin

Product Manual

Leptin is a 16 kDa, 146 amino acid residue non-glycosylated protein hormone involved in regulating body weight, metabolism and reproductive function. It is encoded by the obese (ob) gene and expressed predominantly by adipocytes consistent with the fact that body weight is sensed mainly as the total mass of fat in the body. Leptin is also secreted by cells in the epithelium of the stomach and in the placenta in smaller amounts. Leptin receptors are highly expressed in areas of the hypothalamus that are known to be important in regulating body weight, as well as in T lymphocytes and vascular endothelial cells.

 

 

Recombinant Human or Mouse Leptin (OB)

 

Catalog Number Size Price

L1102S-Human 2.0mg $298.00

L1102L-Human 5.0mg $448.00

L1103S-Mouse 1.0mg $148.00

L1103L-Mouse 10.0mg $898.00

 

*Please call for quote if larger sizes needed.

 

Formulation: Powder lyophilized from volatile buffer 50mM NH4HCO3 (pH 8.0).

Preservative: None.

MW: 16kDa

Purity: >97% (15% SDS-PAGE)

Source: Recombinant protein expressed and purified from E. coli.

Sterility: 0.2 m membrane-filtered and packaged aseptically.

ED50: ND

Endotoxin: 0.1 EU/mg (determined by Limulus Amebocyte Lysate (LAL) Assay

QC Tests: SDS-PAGE, Native PAGE

Product

RNA Interference

Dominant Negatives

Protein Labeling

Agar Plates

Service

Cloning

Mutagenesis

Resource

Vectors

Manuals

Literature

Contact Us

 

Phone:(858) 457-3658

Fax:(858) 457-3485

Emails

Info@biomyx.net

Orders@biomyx.net

 

 

Reconstitution and Use:

Reconstitute the contents of the vial using a sterile buffer (pH8.0 or above) to a concentration no less than 100 mg/ml and aliquot for future use. (If the initial rehydration is too dilute, activity may be lost due to the non-specific adsorption to the container). The solution can then be further diluted to a working stock solution.

 

If the product is going to be used for applications requiring absolute asepsis, its best to filter-sterilize the solution using a sterile and non-pyrogenic 0.2 m membrane before use.

Storage and Stability:

Upon receiving, store the product at -20C. After reconstitution, store the working aliquots at 2-8 C for no more than 3 months. For extended storage, aliquot the rehydrated solution (100mg/ml) and freeze at -70C or -20C. Avoid repeated freezing and thawing. Dilute solutions stored at -20C could lose activity faster.

Further Information:

Cherhab FF, Mounzih K, Lu R, Lim ME: Early onset of reproductive function in normal female mice treated with leptin. Science 275:88, 1997.

Clement K, Vaisse C, Lahlou N, et al: A mutation in the human leptin receptor gene causes obesity and pituitary dysfunction. Nature 392:398, 1998.

Considine RV, Sinha MK, Heiman ML etc: Serum immunoreactive-leptin concentrations in normal-weight and obese humans. New Eng J Med 334:292, 1996.

Friedman JM, Halaas JL: Leptin and the regulation of body weight in mammals. Nature 395:763, 1998.

Halaas JL, Gajiwala KS, Maffel M, etc: Weight-reducing effects of the plasma protein encoded by the obese gene. Science 269:543, 1995.

Montague CT, Faroozi IS, Whitehead JP, etc: Congenital leptin deficiency is associated with severe early-onset obesity in humans. Nature 387:903, 1997.

Pelleymounter MA, Cullen MJ, Baker MB, etc: Effects of the obese gene product on body weight regulation in ob/ob mice. Science 269:540, 1995.

Zhang Y, Proenca R, Maffei M, etc: Positional cloning of the mouse obese gene and its human homologue. Nature 372:425, 1994.).

 

 

 

 

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Human Leptin, Mouse Leptin are supplied lyophilized. Human Leptin and Mouse Leptin peptide sequences are available.