Human/mouse IL6, human and mouse interleukin-6 and interleukin 6

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Interleukin-6

Human or Mouse

Interleukin-6 (IL-6) is a pro-inflammatory cytokine that also has an important role in immunity. IL-6 induces growth and terminal differentiation of B cells; secretion of immunoglobulins; differentiation and activation of T cells and macrophages; and the induction of acute-phase response proteins. Many types of cells, including macrophages, T cells, fibroblasts, and endothelial cells, produce IL-6 in response to stimuli such as bacteria, viruses, and other cytokines, particularly IL-1 and tumor necrosis factor, alpha (TNF). Human IL-6 is active on both mouse and rat cells, while mouse IL-6 has no activity on human cells. Recombinant human IL-6 is a 22 kDa protein containing 189 amino acid residues

Products

Catalog Number

Source

Size

Price

I1005-20	Human	20μg	$149.50
I1005-200	Human	200μg	$989.50
I1005-1000	Human	1000μg	$2499.50

I1006-20	Mouse	20μg	$149.50
I1006-200	Mouse	200μg	$989.50
I1006-1000	Mouse	1000μg	$2499.50

Formulation: Lyophilized powder lyophilized from a volatile buffer (50 mM NH₄HCO₃ , pH 8.0).

Preservative: None.

MW: 21 kD

Purity: >97% on 15% SDS-PAGE.

Source: Recombinant mature protein expressed in E. coli (189 amino acid residues).

Sterility: 0.2 µm membrane-filtered and packaged aseptically.

ED50: ND

Endotoxin*: ≤0.1 EU/mg. Endotoxin Unit is determined by Limulus Amebocyte Lysate (LAL) assay (Sigma)

Receiving and Storage

Upon receiving, store the product at -20^oC. After reconstitution, store the working aliquots at 2-8^oC for no more than 3 months. For extended storage, aliquot the solution (≥100μg/ml) and freeze at -70^oC or -20^oC. Avoid repeated freezing and thawing. More dilute solutions stored at -20^oC will lose activity faster.

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Reconstitution and Use

Reconstitute the contents of the vial using sterile phosphate-buffered saline (PBS) to a concentration no less than 100μg/ml and aliquot for future use. (If the initial rehydration is too dilute, activity may be lost due to the non-specific adsorption to the container). The solution can then be further diluted to a working stock solution.

If the product is going to be used for applications requiring absolute asepsis, it’s best to filter-sterilize the solution using a sterile and non-pyrogenic 0.2µm membrane before use.

References

Kishimoto, T. (1989) Blood 74:1.

Barton, B.E. (1997) Clin. Immunol. Immunopathol. 85:16.

Poupart, P. et al. (1987) EMBO J. 6:1219.

Hibi, M. et al. (1996) J. Mol. Med. 74:1.

Hirano, T. et al. (1994) Stem Cells 12:262.

Akira, S. et al. (1993) Adv. Immunol. 54:1.

Van Snick, J. (1990) Annu. Rev. Immunol. 8:253.

Kishimoto, T. et al. (1992) Polyfunctional Cytokines: IL-6 and LIF. Wiley, Chichester (CIBA Found Symp 167) p. 5.

Kishimoto, T. (1992) Int. Arch. Allergy Immunol. 99:172.

Chiu, C-P. et al. (1988) Proc. Natl. Acad. Sci. USA 85:7099.

Northemann, W. et al. (1989) J. Biol. Chem. 264:16072

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Human and mouse interleukin-6, IL-6, interleukin 6, IL6 or IL-6.