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Product Index

¨     Protein Expression

Mammalian expression vectors with H1 or U6 promoter

Expression vectors with other promoters

ProtéGeneTM expression ready, gene-mutant collection

Wildtype, Dominant Negative and Constitutively Active isoforms

¨     Signal Transduction/Reporter Vectors

StimulightTM Reporter

¨     Media for Bacterial and Yeast Growth

Redi-PlateTM

Liquid Media

¨     Services

Cloning

Mutagenesis

¨     Protein Labeling

ProLinkTM

¨      Groth Factors/Protein Kinase Substrate

TNF-a

Human/Mouse IL6

Human/Mouse Leptin

Mouse IkBa, purified protein

Human cJun, purified protein kinase substrate/TNF-a/TNF a

 

ă 2005 Biomyx Technology · 10054 Mesa Ridge Court Ste. 112 · San Diego · CA 92121

 

Biomyx Product List

 

The following links will help you find the products of your interest. Using Ctrl+F to search will also make finding products easier.

Growth Factors

StimulightTM Reporter Vectors With cis-Elements

Mammalian protein and RNA expression vectors

ProtéGeneTM Dominant Negatives and Constitutively Actives

Prepoured and Custom Agar Plates

 

 

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Growth Factors

Product Name and Descriptions

Cat#                   Unit           List Price

TNF-a

Recombinant Human Tumor Necrosis Factor Alpha

(TNF-alpha)

T1100-20         20mg        $299.00

T1100-200       200mg       $750.00

T1100-1000     1.0mg      $1,999.00

hIL-6

Recombinant Human Interleukin 6

I1005-20         20mg        $149.50

I1005-200       200mg       $989.50

I1005-1000      1.0mg      $2,499.50

mIL-6

Recombinant Mouse Interleukin 6

I1006-20         20mg        $149.50

I1006-200       200mg       $989.50

I1006-1000      1.0mg      $2,499.50

Human Leptin

Recombinant Human Leptin (OB)

L1102S            2mg         $298.00

L1102L            5mg         $448.00

Mouse Leptin

Recombinant Mouse Leptin (OB)

L1103S            1mg         $148.00

L1103L            10mg       $898.00

Murine IκBα

Recombinant murine inhibitor of NFκB alpha, IkBa of aa reidues 1-93

I1009S            0.1mg      $249.00

I1009L             1mg         $1,999.00

Human cJun

Human c-Jun purified to >90%. Optimal phoshporylation substrate of JNK.

J1100S            100μg      $249.00

J1100L            1mg         $1,999.00

 

 

 

StimulightTM Reporter Vectors With cis-Elements

Kit Contents: 40μg cis-containing vector and corresponding pHTS-MCS hygromycin or pHTS-MCS neomycin cloning vector as negative control.

 

 

pHTS-CRE

cAMP luciferase reporter for making stable cell lines with hygromycin selection marker.

Catalog#

Amount

Price

P2100

40mg

$450.00

pHTS-Neo-CRE

cAMP luciferase reporter for making stable cell lines with neomycin (G418) selection marker.

Catalog#

Amount

Price

P2102

40mg

$450.00

 

 

 

pHTS-GAS

g-interferon luciferase reporter for making stable cell lines with hygromycin selection marker.

Catalog#

Amount

Price

P2105

40mg

$450.00

pHTS-Neo-GAS

g-interferon luciferase reporter for making stable cell lines with neomycin (G418) selection marker.

Catalog#

Amount

Price

P2107

40mg

$450.00

 

 

 

pHTS-ISRE

a,b-interferon luciferase reporter for making stable cell lines with hygromycin selection marker.

Catalog#

Amount

Price

P2110

40mg

$450.00

pHTS-Neo-ISRE

a,b-interferon luciferase reporter for making stable cell lines with neomycin (G418) selection marker.

Catalog#

Amount

Price

P2112

40mg

$450.00

 

 

 

pHTS-NFAT

NFAT luciferase reporter for making stable cell lines with hygromycin selection marker.

Catalog#

Amount

Price

P2115

40mg

$450.00

pHTS-Neo-NFAT

NFAT luciferase reporter for making stable cell lines with neomycin (G418) selection marker.

Catalog#

Amount

Price

P2117

40mg

$450.00

 

 

 

pHTS-NFkB

NFkB luciferase reporter for making stable cell lines with hygromycin selection marker.

Catalog#

Amount

Price

P2120

40mg

$450.00

pHTS-Neo-NFkB

NFkB luciferase reporter for making stable cell lines with neomycin (G418) selection marker.

Catalog#

Amount

Price

P2122

40mg

$450.00

 

 

 

pHTS-AP1

Activation by AP-1 complex (c-Jun/fos) with luciferase reporter for making stable cell lines with hygromycin selection marker.

Catalog#

Amount

Price

P2125

40mg

$450.00

pHTS-Neo-AP1

Activation by AP-1 complex (c-Jun/fos) with luciferase reporter for making stable cell lines with neomycin (G418) selection marker.

Catalog#

Amount

Price

P2127

40mg

$450.00

 

 

 

pHTS-MCS

cis-enhancers or cis-responsive elements cloning vector with hygromycin selection marker. with luciferase reporter and hygromycin coding sequence for stable cell line selection

Catalog#

Amount

Price

P2130

40mg

$450.00

pHTS-MCS-Neo

cis-enhancers or cis-responsive elements cloning vector with hygromycin selection marker. with luciferase reporter and neomycin coding sequence for stable cell line selection with G418

Catalog#

Amount

Price

P2132

40mg

$450.00

 

 

 

 

Products for mammalian expression

pMEV

Mammalian expression vector designed for high level expression of proteins in mammalian cells using the strong CMV promoter and optimized 5’-leader sequences.

Catalog#

Amount

Price

P1000

20mg

$195.00

pMEV-βGal

Control expression vector designed for high level expression of β-Gal in mammalian cells using the strong CMV promoter and optimized 5’-leader sequences.

Catalog#

Amount

Price

P2800

40mg

$150.00

 

 

 

pMEV-2HA

pMEV-2HA vectors are designed for high level protein expression in mammalian cells as HA fusions. The use of 2xHA tags increases the detection sensitivity with anti-HA antibodies.

Catalog#

Amount

Price

P1001

20mg

$298.00

P36 Primer

N-terminal sequencing primer for pMEV and pMEV-2HA expression vectors. Sufficient to prepare 250ml of 20mM primer solution. 5'-ccgtcagatcactagaagct-3'

Catalog#

Amount

Price

P1000a

0.5nmole

$95.00

P43 Primer

C-terminal sequencing primer for pMEV and pMEV-2HA expression vectors. Sufficient to prepare 250ml of 20mM primer solution. 5'-cttatcatgtctgctcgaagc-3'

Catalog#

Amount

Price

P1000b

0.5nmole

$95.00

 

 

 

pU6-siRNA

Mammalian short RNA expression vector with mouse U6 small nuclear RNA promoter. Kit includes 20mg of pU6 plasmid (0.5mg/ml); 0.5nmole U6 Sequencing Primer, (Part# P8018B: 10pmole/ml $95.00)

Catalog#

Amount

Price

P8018

20mg

$195.00

 

 

 

pH1-siRNA

Mammalian short RNA expression vector with human H1 small nuclear RNA Promoter. Kit includes 20mg of pH1 (0.5mg/ml) Plasmid; 0.5nmole of H1 Sequencing Primer, (Part# P8020B: 10pmole/ml $95.00)

Catalog#

Amount

Price

P8020

20mg

$195.00

 

 

 

Short RNA Expression Vector Kit

Mammalian small nuclear RNA expression vector kit complete with components in catalog numbers P8018 and P8020.

Catalog#

Amount

Price

P8019

20mg each

$315.00

 

 

 

U6 Sequencing Primer

For sequencing inserts in pU6-siRNA vector. Sufficient to prepare 250ml of 20mM primer solution.

Catalog#

Amount

Price

P8018B

0.5nmole

$95.00

 

 

 

H1 Sequencing Primer

For sequencing inserts in pU6-siRNA vector. Sufficient to prepare 250ml of 20mM primer solution.

Catalog#

Amount

Price

P8020B

0.5nmole

$95.00

 

ProtéGeneTM Dominant Negatives and Constitutively Actives

 

ProtéGeneTM clones complete with wild-type genes and their mutants are fully sequenced and expression-verified. Transfection quality supercoiled plasmids are provided ready to start your experiments. If the gene or the mutant of the gene you are interested in cannot be found on the list, please inquire about our custom cloning and mutagenesis services.

 

These ProtéGeneTM expression sets are currently available:

HARAS, NRAS, KRAS2A, KRAS2B, MEK1, P53, MEKK1, CRAF1, BRAF, P38, JNK1, IκBα, JNKK1, JNKK2,

 

 

 

 

Human HARAS Set

Expression-ready clones of human Ha-Ras.Kit includes: pMEVHA-HRAS-WT

pMEVHA-HRAS-S17N

pMEVHA-HRAS-G12V

Catalog#

Amount

Price

P1010

20mg each

$995.00

pMEVHA-HRAS-WT

Expresses human HRAS1 wild type

Catalog#

Amount

Price

P1010a

20mg

$565.00

pMEVHA-HRAS-S17N

Expresses a dominant negative mutant of human HRAS1 (S17N)

Catalog#

Amount

Price

P1010b

20mg

$565.00

pMEVHA-HRAS-G12V

Expresses a constitutively active mutant of human HRAS1 (G12V)

Catalog#

Amount

Price

P1010c

20mg

$565.00

 

 

 

Human NRAS Set

Expression-ready clones of human N-Ras. Kit includes: pMEVHA-NRAS-WT

pMEVHA-NRAS-S17N

pMEVHA-NRAS-G12V

Catalog#

Amount

Price

P1015

20mg each

$995.00

pMEVHA-NRAS-WT

Expresses wild type human NRAS1

Catalog#

Amount

Price

P1015a

20mg

$565.00

pMEVHA-NRAS-S17N

Expresses a dominant negative mutant of human NRAS1 (S17N)

Catalog#

Amount

Price

P1015b

20mg

$565.00

pMEVHA-NRAS-G12V

Expresses a constitutively active mutant of human NRAS1 (G12V)

Catalog#

Amount

Price

P1015c

20mg

$565.00

 

 

 

Human KRAS2A Set

Expression-ready clones of human KRAS2A. Kit includes: pMEVHA-KRAS2A-WT

pMEVHA-KRAS2A-S17N

pMEVHA-KRAS2A-G12V

Catalog#

Amount

Price

P1020

20mg each

$995.00

pMEVHA-KRAS2A-WT

Expresses wild type human KRAS2A1

Catalog#

Amount

Price

P1020a

20mg

$565.00

pMEVHA-KRAS2A-S17N

Expresses a dominant negative mutant of human KRAS2A1 (S17N)

Catalog#

Amount

Price

P1020b

20mg

$565.00

pMEVHA-KRAS2A-G12V

Expresses a constitutively active mutant of human KRAS2A1 (G12V)

Catalog#

Amount

Price

P1020c

20mg

$565.00

 

 

 

Human KRAS2B Set

Expression-ready clones of human KRAS2B. Kit includes: pMEVHA-KRAS2B-WT

pMEVHA-KRAS2B-S17N

pMEVHA-KRAS2B-G12V

Catalog#

Amount

Price

P1025

20mg each

$995.00

pMEVHA-KRAS2B-WT

Expresses wild type human KRAS2B1

Catalog#

Amount

Price

P1025a

20mg

$565.00

pMEVHA-KRAS2B-S17N

Expresses a dominant negative mutant of human KRAS2B1 (S17N)

Catalog#

Amount

Price

P1025b

20mg

$565.00

pMEVHA-KRAS2B-G12V

Expresses a constitutively active mutant of human KRAS2B1 (G12V)

Catalog#

Amount

Price

P1025c

20mg

$565.00

 

 

 

Human MEK1 Set

Expression-ready clones of human MEK1

pMEVHA-MEK1-WT, pMEVHA-MEK1-CA, pMEVHA-MEK1-DN, pMEVHA-MEK1-K97R

Catalog#

Amount

Price

P1030

20mg each

$1355.00

pMEVHA-MEK1-WT

Expresses wild type human MEK1

Catalog#

Amount

Price

P1030a

20mg

$565.00

pMEVHA-MEK1-DN

Expresses a dominant negative mutant of human MEK1 (K97R; S218A; S222A)

Catalog#

Amount

Price

P1030b

20mg

$565.00

pMEVHA-MEK1-CA

Expresses a constitutively active mutant of human MEK1 (Delta aa32-51; S218E; S222E)

Catalog#

Amount

Price

P1030c

20mg

$565.00

pMEVHA-MEK1-K97R

Expresses a kinase deficient mutant of humane MEK1 (K97R)

Catalog#

Amount

Price

P1030d

20mg

$565.00

 

 

 

Human P53 Set

Expression-ready clones of human tumor suppressor p53. Kit includes: pMEVHA-P53-WT, pMEVHA-P53-DN

Catalog#

Amount

Price

P1035

20mg each

$865.00

pMEVHA-P53-WT

Expresses wild type human p53

Catalog#

Amount

Price

P1035a

20mg

$565.00

pMEVHA-P53-DN

Expresses a dominant negative mutant of human P53 (V143A)

Catalog#

Amount

Price

P1035b

20mg

$565.00

 

 

 

Mouse MEKK1 Set

Expression-ready clones of mouse MEKK1:

pMEVHA-MEKK1-KD (CA)

pMEVHA-MEKK1-K452M

Catalog#

Amount

Price

P1045

20mg each

$865.00

pMEVHA-MEKK1-KD

Expresses the kinase domain of wild type mouse MEKK1, amino acid residues 375-672 . It’s constitutively active in mammalian cells

Catalog#

Amount

Price

P1045a

20mg

$565.00

pMEVHA-MEKK1-K452M

Expresses the kinase domain (375-672) of a mutant mouse MEKK1 (K452M). It lacks protein kinase activity.

Catalog#

Amount

Price

P1045b

20mg

$565.00

 

 

 

pMEVHA-CRAF1-KD

Expresses the kinase domain of wild type human RAF1, amino acid residues 305-648.

Catalog#

Amount

Price

P1040

20mg

$565.00

 

 

 

Human BRAF Set

Expression ready clones of human BRAF

Catalog#

Amount

Price

P1060

20mg each

$1356.00

pMEVHA-BRAF-WT

Expresses wild type human BRAF

Catalog#

Amount

Price

P1060a

20mg

$565.00

pMEVHA-BRAF-K482M

Expresses the kinase deficient human BRAF

 

Catalog#

Amount

Price

P1060b

20mg

$565.00

pMEVHA-BRAF-ED

Expresses constitutively active human BRAF, T598E, S601D

Catalog#

Amount

Price

P1060c

20mg

$565.00

pMEVHA-BRAF-V599E

Expresses natural oncogenic mutant human BRAF, V599E

Catalog#

Amount

Price

P1060d

20mg

$565.00

 

 

 

Human P38 Expression Set

Expression-ready clones of human P38 (MAPK14) set

Catalog#

Amount

Price

P1070

20mg each

$1356.00

pMEVHA-P38-wt

Expression-ready clone of human P38 (MAPK14) wild type

Catalog#

Amount

Price

P1070a

20mg

$565.00

pMEVHA-P38-K53M

Expression-ready clone of human P38 (MAPK14), kinase deficient mutant

Catalog#

Amount

Price

P1070b

20mg

$565.00

pMEVHA-P38-EE
Expression-ready clone of human P38 mutant with the activation phosphorylation sites T180 and Y182 replaced with glutamate.

Catalog#

Amount

Price

P1070c

20mg

$565.00

pMEVHA-P38-AA
Expression-ready clone of human P38 mutant with the activation phosphorylation sites T180 and Y182 replaced with alanine.

Catalog#

Amount

Price

P1070d

20mg

$565.00

 

 

 

Human JNK1 Set

Expression-ready clones of human JNK1

Catalog#

Amount

Price

J1105

20mg each

$1356.00

pMEVHA-JNK1wt

Expresses the wild type human JNK1

Catalog#

Amount

Price

J1105a

20mg

$565.00

pMEVHA-JNK1-K55M

Expresses the kinase deficient human JNK1

Catalog#

Amount

Price

J1105b

20mg

$565.00

pMEVHA-JNK1-EE

Expression-ready clone of human JNK1 mutant with the activation phosphorylation sites T183 and Y185 replaced with glutamate

Catalog#

Amount

Price

J1105c

20mg

$565.00

pMEVHA-JNK1-AA

Expression-ready clone of human JNK1 mutant with the activation phosphorylation sites T183 and Y185 replaced with alanine

Catalog#

Amount

Price

J1105d

20mg

$565.00

 

 

 

Human IκBα Expression Set

Expression-ready clones of human IκBα set

Catalog#

Amount

Price

I1000

20mg each

$865.00

pMEVHA-IκBα-wt

Expresses the wild type human IκBα

Catalog#

Amount

Price

I1000a

20mg

$565.00

pMEVHA-IκBα-S(32, 36)A

Expresses the AA mutant form of  human IκBα (S32A, S36A)

Catalog#

Amount

Price

I1000a

20mg

$565.00

 

 

 

Human JNKK1 Set

Expression-ready clones of human JNKK1 (MAP2K4)

Catalog#

Amount

Price

J1106

20mg each

$1356.00

pMEVHA-JNKK1-wt

Expresses the wild type human JNKK1 (MAP2K4).

Catalog#

Amount

Price

J1106a

20mg

$565.00

pMEVHA-JNKK1-K131M

Expresses the kinase deficient mutant of human JNKK1 (MAP2K4).

Catalog#

Amount

Price

J1106b

20mg

$565.00

pMEVHA-JNKK1-EE

Expresses the human JNKK1 (MAP2K4) mutant with the phosphorylation activation sites S257 and T030 mutated to glutamates.

Catalog#

Amount

Price

J1106c

20mg

$565.00

pMEVHA-JNKK1-AA

Expresses the human JNKK1 (MAP2K4) mutant with the phosphorylation activation sites S257 and T030 mutated to alanines.

Catalog#

Amount

Price

J1106d

20mg

$565.00

 

 

 

Human JNKK2 Expression Set

Expression-ready clones of human JNKK2 set

Catalog#

Amount

Price

J1108

20mg each

$1356.00

pMEVHA-JNKK2-wt

Expresses the wild type human JNKK2 (MAP2K7)

Catalog#

Amount

Price

J1108a

20mg

$565.00

pMEVHA-JNKK2-K149M

Expresses the Kinase deficient mutant of human JNKK2 (MAP2K7)

Catalog#

Amount

Price

J1108b

20mg

$565.00

pMEVHA-JNKK2-EE

Expresses the human JNKK2 (MAP2K7) with the activation phosphorylation sites S271 and T275 mutated to glutamates.

Catalog#

Amount

Price

J1108c

20mg

$565.00

pMEVHA-JNKK2-AA

Expresses the human JNKK2 (MAP2K7) with the activation phosphorylation sites S271 and T275 mutated to alanines.

Catalog#

Amount

Price

J1108d

20mg

$565.00

 

 

 

 

Prepoured and Custom Agar Plates

 

Lauria-Bertani (LB) agar plates are prepared with the highest quality ingredients and most stringent quality testing for reproducible growth results.

 

List prices are for sleeve of 10 plates. EZ-Spreader™ spreading beads included for easy and safe plating of bacteria.

 

Description

Catalog Number

Price

 

 

 

EZ-SpreaderTM: 10 vials of sterile beads for plating 60-100 Plates.

S-1050S

$9.00

EZ-SpreaderTM: One tube of sterile beads for plating 120-200 Plates.

S-1050L

$12.00

LB Agar, 100x15mm

LB-1100

$15.00

LB Agar, 150x15mm

LB-1150

$20.00

LB Agar, 50mg/ml Ampicillin, 100x15mm

LA-1100

$15.00

LB Agar, 100mg/ml Ampicillin, 100x15mm

LA-2100

$15.00

LB Agar, 50mg/ml Ampicillin, 0.1mM IPTG, 60mg/ml X-gal, 100x15mm

LA-3100

$16.00

LB Agar, 75mg/ml Carbenicillin, 100x15mm

LA-4100

$15.00

LB Agar, 75mg/ml Carbenicillin, 0.1mM IPTG, 60mg/ml X-gal, 100x15mm

LA-5100

$16.00

LB Agar, 50mg/ml Ampicillin, 150x15mm

LA-1150

$20.00

LB Agar, 100mg/ml Ampicillin, 150x15mm

LA-2150

$20.00

LB Agar, 50mg/ml Ampicillin, 0.1mM IPTG, 60mg/ml X-gal, 150x15mm

LA-3150

$22.00

LB Agar, 75mg/ml Carbenicillin, 150x15mm

LA-4150

$20.00

LB Agar, 75mg/ml Carbenicillin, 0.1mM IPTG, 60mg/ml X-gal, 150x15mm

LA-5150

$22.00

LB Agar, 34 mg/ml Chloramphenicol, 100x15mm

LC-1100

$15.00

LB Agar, 34 mg/ml Chloramphenicol, 150x15mm

LC-1150

$20.00

LB Agar, 50mg/ml Kanamycin, 100x15mm

LK-1100

$15.00

LB Agar, 50mg/ml Kanamycin, 150x15mm

LK-1150

$20.00

LB Agar, 35mg/ml Tetracycline, 100x15mm

LT-1100

$15.00

LB Agar, 35mg/ml Tetracycline, 150x15mm

LT-1150

$20.00

LB Agar, 50mg/ml Ampicillin, 25mg/ml Kanamycin, 100x15mm

LD-1100

$16.00

LB Agar, 50mg/ml Ampicillin, 34mg/ml Chloramphenicol, 100x15mm

LD-2100

$16.00

LB Agar, 50mg/ml Ampicillin, 35mg/ml Tetracycline, 100x15mm

LD-3100

$16.00

2xYT plates, 100x15mm

YT-1100

$16.00

2xYT plates, 100mg/ml Ampicillin, 100x15mm

YT-1101

$16.00

 

 

 

Custom Agar Plate: Agar plates to your specifications, 100x15mm

CM-100

Inquire

Custom Agar Plate: Agar plates to your specifications, 150x15mm

CM-150

Inquire

 

Liquid Media

Description

Unit Size

Catalog Number

Price

Sterile Liquid LB medium (Miller)

100ml

LB-100

$10.00

Sterile Liquid LB medium (Miller)

250ml

LB-250

$14.00

Sterile Liquid LB medium (Miller)

1000ml

LB-1000

$28.00

Sterile Liquid 2xYT Medium

100ml

YT-100

$12.00

Sterile Liquid 2xYT Medium

250ml

YT-250

$18.00

Sterile Liquid 2xYT Medium

1000ml

YT-1000

$36.00

Sterile Liquid Terrific Broth Medium

1000ml

TB-1000

$36.00

SOC medium (for transformation)

100ml

SOC-100

$20.00

 

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