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Manuals and Product Information Sheets

Index

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pH1-siRNA, pU6-siRNA, pHTS Reporter Vectors, pMEV, pMEV-2HA, Human MEK1, Human BRAF, Mouse MEKK1, Human P53, Human H-RAS, Human K-RAS2A, Human IkBa, Human c-Jun Purified Protein, Human N-RAS, Human K-RAS2B, Human JNK1, Human P38, Human AKT1

pH1-siRNA

Cat#: P8020

Map and features of pH1-siRNA vector for transient or stable short interfering RNA (siRNA) expression in mammalian cells for RNA interference (RNAi) to knock down protein expression level. It also includes information and examples on how to design and clone siRNA-encoding oligos with stem-loop or hairpin structure. Vector Sequence

pU6-siRNA

Cat#: P8018

Map and features of pU6-siRNA vector for transient or stable short interfering RNA (siRNA) expression in mammalian cells for RNA interference (RNAi) to knock down protein expression level. It also includes information and examples on how to design and clone siRNA-encoding oligos with stem-loop or hairpin structure. Vector Sequence

pHTS Reporter Vectors

Cat#: P2130, P2100, P2015, P2110, P2115, P2120

Covers group of vectors covers signal transduction pathways converging at several common enhancer elements including CRE (Cat# P2100, cyclic AMP response element), GAS (P2105, interferon gĖactivated sequence), ISRE (P2110, interferon-stimulated response element), AP1 (P2125, activator protein 1), NFAT (P2115, nuclear factor of activated T cells) and NFkB (P2120, binding sites for nuclear factor kB). A cloning vector (pHTS-MCS, Cat# P2130 with Hygro resistance, and P2132 with Neo resistance) is also available so that one can create synthetic promoters to monitor new signaling pathways of interest. Click on links for vector sequences.

pMEV

Cat#: P1000

Mammalian expression vector designed for high level expression of proteins in mammalian cells in its native form using the strong CMV promoter and optimized 5í-leader sequences.

It has no expression tag (no fusion tag). Vector Sequence

pMEV-2HA

Cat#: P1001

pMEV-2HA mammalian expression vectors (frames A, B and C) are designed for high-level expression of proteins in mammalian cells using the strong CMV promoter and optimized 5í-leader sequences. The use of 2xHA tags increases the detection sensitivity with anti-HA antibodies. Vector Sequence

Human MEK1 Set

Cat#: P1030

Expression-ready clones of human MEK1:

pMEVHA-MEK1-WT, wild type human MEK1

pMEVHA-MEK1-DN, dominant negative MEK1

pMEVHA-MEK1-CA, constitutively active MEK1

pMEVHA-MEK1-K97R, kinase deficient MEK1 (K97R)

Human BRAF Set

Cat#: P1060

Expression-ready clones of human BRAF. Set includes:

pMEVHA-BRAF-WT, pMEVHA-BRAF-K482M

pMEVHA-BRAF-ED, pMEV-BRAF-V599E

Mouse MEKK1 Set

Cat#: P1045

Expression-ready clones of mouse MEKK1

pMEVHA-MEKK1-KD (CA)

pMEVHA-MEKK1-K452M

Human P53 Set

Cat#: P1035

Expression-ready clones of human tumor suppressor p53. Kit includes:

pMEVHA-P53-WT, wild type p53, pMEVHA-P53-DN, dominant negative p53

Human H-RAS Set

Cat#: P1010

Expression-ready clones of human H-Ras. Kit includes:

pMEVHA-HRAS-WT, wild type H-Ras; pMEVHA-HRAS-S17N, dominant negative H-Ras

pMEVHA-HRAS-G12V, constitutively active H-Ras

 

Human KRAS2A Set

Cat#: P1020

Expression-ready clones of human K-Ras2A. Kit includes:

pMEVHA-KRAS2A-WT, wild type K-Ras2A

pMEVHA-KRAS2A-S17N, dominant negative K-Ras2A

pMEVHA-KRAS2A-G12V, constitutively active K-Ras2A

Human IκBα Set

Cat#: I1000

Expression-ready clones of Human IκB alpha (IkBa). Set includes:

pMEVHA-IκBα wildtype and AA muntant form pMEVHA-IκBα-S(32,36)A

 

Human c-Jun, Purified Protein

Cat#: J1100S

J1100L

Purified (>90%) recombinant human c-Jun (1-79) serves as a highly specific substrate for c-Jun N-terminal kinases (JNK) due to the presence of a JNK docking region. Itís not phosphorylated by ERKs and p38 MAPKs. Therefore, itís a preferred substrate for in vitro JNK activity assays.

Murine GST-IKBa
Cat#I1009S, I1009L

Recombinant murine inhibitor of NFkB alpha (IkBa), amino acid residues 1-93

 

pKG-cJun

Cat#: P6050

Bacterial expression clone that expresses human c-Jun (1-79) in fusion with GST tag to facilitate easy one-step purification.

Human N-RAS Set

Cat#: P1015

Expression-ready clones of human N-Ras. Set includes:

pMEVHA-NRAS-WT, wild type N-Ras

pMEVHA-NRAS-S17N, dominant negative N-Ras

pMEVHA-NRAS-G12V, constitutively active N-Ras

 

Human KRAS2B Set

Cat#: P1025

Expression-ready clones of human K-Ras2B. Set includes:

pMEVHA-KRAS2B-WT, wild type human K-Ras2B

pMEVHA-KRAS2B-S17N, dominant negative K-Ras2B

pMEVHA-KRAS2B-G12V, constitutively active K-Ras2B

 

Human JNK1 Set

Cat#: J1105

Expression-ready clones of human JNK1. Set includes:

pMEVHA-JNK1-WT

pMEVHA-JNK1-K55M

 

Human P38 Set

Cat#: P1070

Expression-ready clones of human p38. Set includes:

pMEVHA-P38-WT, pMEVHA-P38-K53M

 

Human P38G Set
Cat#P1072

Expression-ready clones of human P38 gamma (MAPK12). Set includes: pMEVHA-P38G-WT, pMEVHA-P38G-K56M, pMEVHA-P38G-EE and pMEVHA-P38G-AA

 

Human JNKK1 Set
Cat#J1106

Expression-ready clones of human JNKK1 (MAP2K4). Set includes: pMEVHA-JNKK1-WT, pMEVHA-JNKK1-K131M, pMEVHA-JNKK1-EE and pMEVHA-JNKK1-AA

 

Human JNKK2 Set
Cat#J1108

Expression-ready clones of human JNKK2 (MAP2K7). Set includes: pMEVHA-JNKK2-WT, pMEVHA-JNKK2-K149M, pMEVHA-JNKK2-EE and pMEVHA-JNKK2-AA

 

Human AKT1 Set
Cat#A1010

Expression-ready clones of human AKT1. Set includes:pMEVHA-AKT1-WT, pMEVHA-AKT1-K179A, pMEVHA-AKT1-DD, pMEVHA-AKT1-AA and pMEVHA-AKT1-KAAA

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