|
|
|
ProtéGeneä Expression Ready Clones for Stemness Genes |
|
|
|
|
|
Some
genes such as OCT3/4, SOX2, NANOG, LIN28 and KLF4 are highly expressed in
undifferentiated embryonic stem cells and progenitor cells. They are
important for stem cells to have their special properties or “stemness”, and hence are sometimes called “Stemness Genes”. Tremendous advances have been achieved
recently in induced pluripotent stem (iPS) cells from mouse and human somatic cells. These
cells have enormous potentials in curing many human diseases. To facilitate
stem cell research and exploration, Biomyx now
offer all the “Stemness Genes” that have been used
to induce iPS: OCT3/4, SOX2, NANOG, LIN28, c-Myc and KLF4. OCT3/4, SOX2, c-Myc
and KLF4 were initially used to generate induced pluripotent
stem (iPS) cells from mouse adult cells (Takahashi
& Yamanaka, 2007). The same set of genes were later used to induce iPS cells with human somatic cells (Park et al., 2008)
although a different set of genes consisting OCT3/4, SOX2, NANOG and LIN28
were used by Yu et al (2007) to induce iPS cells
from human cells. Important
Features of Expression-Ready Stemness Genes 1.
Every nucleotide of the inserts
in ProteGene clones is confirmed by DNA
sequencing. The inserts are flanked by
common restriction sites so they can be easily cut out and cloned into another
expression vector (e.g. a viral expression vector) if so desired. 2.
Optimized CMV promotor for high-level constitutive mammalian protein
expression so the vectors can be used directly to express the proteins in
mammalian cells. 3.
N-terminal 2xHA tag allows for
easy immunoprecipitation,
enzyme activity assay and Western analysis 4.
The plasmids are Kanamycin resistance in bacteria and G418 resistance in
mammalian cells. 5.
The plasmid
DNA are highly purified and ready for transfection
of mammalian cells. |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Cat# |
Product
Name |
Brief Description |
Amount |
Price (USD) |
|
|
|
|
|
|
||
|
S1006 |
Stemness Gene Set of
6 |
Full set of 6 Expression-Ready Clones for human
OCT3/4, SOX2, NANOG, LIN28, KLF4 and c-Myc in pMEV-2HA
vector. |
20mg each |
$1798.00 |
|
|
Stemness Gene Set of 4 |
Set of
4 Expression-Ready Clones (selected from human OCT3/4, SOX2, NANOG, LIN28,
KLF4 and c-Myc)
|
20mg each |
$1356.00
|
||
|
S1001 |
pMEVHA-OCT3/4 |
Expression-Ready
Clone of human OCT3/4 Human
POU class 5 homeobox 1 (POU5F1), (NM_002701) |
20mg |
$565.00 |
|
|
S1003 |
pMEVHA-SOX2 |
Expression-Ready
Clone of human SOX2 Human
SRY (sex determining regions Y)- |
20mg |
$565.00 |
|
|
S1005 |
pMEVHA-NANOG |
Expression-Ready
Clone of human NANOG Nanog homeobox (NM_024865) |
20mg |
$565.00 |
|
|
S1007 |
pMEVHA-LIN28 |
Expression-Ready
Clone of human LIN28 Human
lin-28 homolog (NM_024674) |
20mg |
$565.00 |
|
|
S1009 |
pMEVHA-KLF4 |
Expression-Ready
Clone of human KLF4 Human Kruppel-like factor 4 (gut) (NM_004235) |
20mg |
$565.00 |
|
|
S1011 |
pMEVHA-cMYC |
Expression-Ready
Clone of human c-Myc Human
v-myc myelocytomatosis
viral oncogene homolog (NM_002467) |
20mg |
$565.00 |
|
|
MAB1001 |
HA Tag Antibody (Mab) |
Purified
mouse monoclonal antibody (0.4 ml
at 0.25 mg/ml) raised with KLH-conjugated synthetic
peptide CYPYDVPDYAYPYDVPDYA. ELISA: 1:5000; Western: 1:1000 |
100mg |
$198.00 |
|
|
PAB1001 |
HA Tag Antibody (Pab) |
Purified
rabbit polyclonal antibody (0.4 ml
at 0.25 mg/ml) raised with KLH-conjugated synthetic peptide
CYPYDVPDYAYPYDVPDYA ELISA: 1:5000; Western: 1:1000 |
100mg |
$198.00 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
1. Takahashi K. & Yamanaka
S. 2006. Cell, 126(4): 663-76 2. Takahashi K. et al.,
2007, Cell, 131: 861-72 3. Yu J, et al., 2007,
Science, 318: 1917-20 4. Meissner A. et al., 2007. Nat Biotechnol. 25: 1177-81 5. Ramalho-Santos M et al., 2007, Cell stem
Cell, 1: 245-7 6. Park IH, et al., 2008.
Nature, 451: 141-6 7. Aoi T. et al., 2008, Science, Epub 14 Feb. 2008, 10.1126/Science.1154884 8. Lowry WE et al., 2008.
PNAS 9. Nakagawa M., 2008. Nat
Biotechnol. 26: 101-6 |
|||||
|
|
|||||
|
Hippo Pathway Genes & Luciferase
Reporter Vectors |
|||||
|
|
|
|
|
|
|
|
|
|||||
|
Cat# |
Product Name |
Brief Description |
Amount |
Price (USD) |
|
|
GAL4-TEAD Luciferase Reporter System |
Luciferase Reporter System for Hippo
Pathway Readout. This system use
Gal4-TEAD4 fusion protein as the sensor of the activation of Hippo kinase cascade. The transcription factor YAP works together
with Gal4-TEAD4 to activate luciferase expression
from pGBD-Hyg-Luc vector when transfected
into mammalian cells. Activated Hippo kinase
cascade results in the phosphorylation of Although the system can
be used to study Hippo pathway by transient transfection
assays, it is especially useful for making stable cell lines for drug
screening because both vectors (pGal4-TEAD4 and pBGD-Hyg-Luc)
have different antibiotics resistance marker (G418 & Hygromycin,
respectively). Positive and negative
controls are included in the kit. |
Each |
$698.00 |
||
|
H1006 |
pMEVHA-FRMD6 (Expanded) |
Expression-Ready Clone
of human FERM domain containing 6 (FRMD6) (NM_152330). |
20mg |
$565.00 |
|
|
H1008 |
pMEVHA-NF2 (Merlin) |
Expression-Ready Clone
of neurofibromin 2 (merlin,
aka. NF2) (NM_181831) |
20mg |
$565.00 |
|
|
H1010 |
pMEVHA-SAV1 ( |
Expression-Ready Clone
of human |
20mg |
$565.00 |
|
|
H1012 |
pMEVHA-Mst2 (Hippo kinase) |
Expression-Ready Clones
of human serine/threonine kinase
3 (STK3) (STE20 homolog) (NM_006281): pMEVHA-Mst2-Wt & pMEVHA-Mst2-K56R (Kinase-deficient mutant) |
20mg |
$865.00 |
|
|
PAB1011 |
Anti-Mst2 Antibody |
Purified rabbit polyclonal
antibody (0.4 ml at 0.25 mg/ml) ELISA:
1:1000; Western: 1:100-500; Immunohistochemistry:
1:10-50 |
100mg |
$258.50 |
|
|
H1014 |
pMEVHA-Lats2 (wts) |
Expression-Ready Clones
of mouse large tumor suppressor 2 (Lats2) (NM_015771) |
20mg |
$565.00 |
|
|
PAB1012 |
Anti-Lats2 Antibody |
Purified rabbit
polyclonal antibody (0.4 ml at 0.25 mg/ml) ELISA: 1:1000; Western: 1:100-500;
Immunohistochemistry: 1:10-50 |
100mg |
$258.50 |
|
|
H1016 |
pMEVHA-YAP |
Expression-Ready Clones
of human yes-associated protein 2, (AY316529) H1016a: pMEVHA-YAP2-WT H1016b: pMEVHA-YAP2-5SA
(phosphorylation sites mutated) H1016c:
pMEVHA-YAP-5SAdC (phosphorylation-deficient and
nuclear localization domain deletion mutant, dominant negative) |
20mg |
$995.00 |
|
|
H1018 |
pMEVHA-MOB1 |
Human Mps One binder kinase activator-like 1B, NM_018221 |
20mg |
$565.00 |
|
|
A1010 |
Human AKT1 Set |
Expression-Ready Clones
of human AKT1: A1010a: pMEV2HA-AKT1-WT; A1010b:
pMEV2HA-AKT1-K179A; A1010c: pMEV2HA-AKT1-DD; A1010d:
pMEV2HA-AKT1-AA; A1010e: pMEV2HA-AKT1-KAAA |
20mg each |
$1480.00 |
|
|
MAB1001 |
HA Tag Antibody (Mab) |
Purified mouse monoclonal antibody (0.4 ml at 0.25
mg/ml) raised with KLH-conjugated synthetic peptide
CYPYDVPDYAYPYDVPDYA. ELISA: 1:5000; Western: 1:1000 |
100mg |
$198.00 |
|
|
PAB1001 |
HA Tag Antibody (Pab) |
Purified rabbit polyclonal antibody (0.4 ml at 0.25
mg/ml) raised with KLH-conjugated synthetic peptide CYPYDVPDYAYPYDVPDYA
ELISA: 1:5000; Western: 1:1000 |
100mg |
$198.00 |
|
|
| |||||