Dominant negatives, Constitutively active mutants, Wild type and mutant expression clones: Stem cell, IRAK1,IRAK4,BRAF,HRAS,NRAS,KRAS2A,KRAS2B,P53, MEKK1, CRAF1, MEK1 IkB, I kappa B alpha,p38,jnk,jnkk1,jnkk2

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ProtéGeneä Expression Ready Clones for

Stemness Genes

 

 

Some genes such as OCT3/4, SOX2, NANOG, LIN28 and KLF4 are highly expressed in undifferentiated embryonic stem cells and progenitor cells. They are important for stem cells to have their special properties or “stemness”, and hence are sometimes called “Stemness Genes”. Tremendous advances have been achieved recently in induced pluripotent stem (iPS) cells from mouse and human somatic cells. These cells have enormous potentials in curing many human diseases. To facilitate stem cell research and exploration, Biomyx now offer all the “Stemness Genes” that have been used to induce iPS: OCT3/4, SOX2, NANOG, LIN28, c-Myc and KLF4. OCT3/4, SOX2, c-Myc and KLF4 were initially used to generate induced pluripotent stem (iPS) cells from mouse adult cells (Takahashi & Yamanaka, 2007). The same set of genes were later used to induce iPS cells with human somatic cells (Park et al., 2008) although a different set of genes consisting OCT3/4, SOX2, NANOG and LIN28 were used by Yu et al (2007) to induce iPS cells from human cells.

 

Important Features of Expression-Ready Stemness Genes

1.         Every nucleotide of the inserts in ProteGene clones is confirmed by DNA sequencing.  The inserts are flanked by common restriction sites so they can be easily cut out and cloned into another expression vector (e.g. a viral expression vector) if so desired.

2.         Optimized CMV promotor for high-level constitutive mammalian protein expression so the vectors can be used directly to express the proteins in mammalian cells.

3.         N-terminal 2xHA tag allows for easy  immunoprecipitation, enzyme activity assay and Western analysis

4.         The plasmids are Kanamycin resistance in bacteria and G418 resistance in mammalian cells.

5.         The plasmid DNA are highly purified and ready for transfection of mammalian cells.

 

 

 

 

 

 

 

 

 

 

 

Cat#

Product Name

Brief Description

Amount

Price (USD)

 

 

 

 

S1006 

Stemness Gene

Set of 6

Full set of 6 Expression-Ready Clones for human OCT3/4, SOX2, NANOG, LIN28, KLF4 and c-Myc in pMEV-2HA vector.

20mg each

$1798.00

S1004

Stemness Gene

Set of 4

Set of 4 Expression-Ready Clones (selected from human OCT3/4, SOX2, NANOG, LIN28, KLF4 and c-Myc) 

20mg each

$1356.00

S1001

pMEVHA-OCT3/4

Expression-Ready Clone of human OCT3/4

Human POU class 5 homeobox 1 (POU5F1), (NM_002701)

20mg

$565.00

S1003

pMEVHA-SOX2

Expression-Ready Clone of human SOX2

Human SRY (sex determining regions Y)-box 2 (NM_003106)

20mg

$565.00

S1005

pMEVHA-NANOG

Expression-Ready Clone of human NANOG

Nanog homeobox (NM_024865)

20mg

$565.00

S1007

pMEVHA-LIN28

Expression-Ready Clone of human LIN28

Human lin-28 homolog (NM_024674)

20mg

$565.00

S1009

pMEVHA-KLF4

Expression-Ready Clone of human KLF4

Human Kruppel-like factor 4 (gut) (NM_004235)

20mg

$565.00

S1011

pMEVHA-cMYC

Expression-Ready Clone of human c-Myc

Human v-myc myelocytomatosis viral oncogene homolog (NM_002467)

20mg

$565.00

MAB1001

HA Tag Antibody

(Mab)

Purified mouse monoclonal antibody (0.4 ml at 0.25 mg/ml) raised with KLH-conjugated synthetic peptide CYPYDVPDYAYPYDVPDYA. ELISA: 1:5000; Western: 1:1000

100mg

$198.00

PAB1001

HA Tag Antibody

(Pab)

Purified rabbit polyclonal antibody (0.4 ml at 0.25 mg/ml) raised with KLH-conjugated synthetic peptide CYPYDVPDYAYPYDVPDYA ELISA: 1:5000; Western: 1:1000

100mg

$198.00

 

 

 

 

 

 

 

 

 

 

Selected References:

1.       Takahashi K. & Yamanaka S. 2006. Cell, 126(4): 663-76

2.       Takahashi K. et al., 2007, Cell, 131: 861-72

3.       Yu J, et al., 2007, Science, 318: 1917-20

4.       Meissner A. et al., 2007. Nat Biotechnol. 25: 1177-81

5.       Ramalho-Santos M et al., 2007, Cell stem Cell, 1: 245-7

6.       Park IH, et al., 2008. Nature, 451: 141-6

7.       Aoi T. et al., 2008, Science, Epub 14 Feb. 2008, 10.1126/Science.1154884

8.       Lowry WE et al., 2008. PNAS USA, 105: 2883-8

9.       Nakagawa M., 2008. Nat Biotechnol. 26: 101-6

 

 

Hippo Pathway Genes & Luciferase Reporter Vectors

 

 

 

 

 

 

Cat#

Product Name

Brief Description

Amount

Price (USD)

H1001

GAL4-TEAD Luciferase

Reporter System

Luciferase Reporter System for Hippo Pathway Readout.

This system use Gal4-TEAD4 fusion protein as the sensor of the activation of Hippo kinase cascade. The transcription factor YAP works together with Gal4-TEAD4 to activate luciferase expression from pGBD-Hyg-Luc vector when transfected into mammalian cells. Activated Hippo kinase cascade results in the phosphorylation of YAP and its cytoplasmic translocation. Less YAP in the nucleus, in turn, cause reduced luciferase expression while inhibition of Hippo kinase cascade results in elevated luciferase activity. This system is very useful for studying the effects of the various components and for screening inhibitors of the Hippo pathway.

 

Although the system can be used to study Hippo pathway by transient transfection assays, it is especially useful for making stable cell lines for drug screening because both vectors (pGal4-TEAD4 and pBGD-Hyg-Luc) have different antibiotics resistance marker (G418 & Hygromycin, respectively).

 

Positive and negative controls are included in the kit.

Each

$698.00

H1006

pMEVHA-FRMD6

(Expanded)

Expression-Ready Clone of human FERM domain containing 6 (FRMD6) (NM_152330).

20mg

$565.00

H1008

pMEVHA-NF2

(Merlin)

Expression-Ready Clone of neurofibromin 2 (merlin, aka. NF2) (NM_181831)

20mg

$565.00

H1010

pMEVHA-SAV1

(Salvador)

Expression-Ready Clone of human Salvador homolog 1 (NM_021818)

20mg

$565.00

H1012

pMEVHA-Mst2

(Hippo kinase)

Expression-Ready Clones of human serine/threonine kinase 3 (STK3) (STE20 homolog) (NM_006281): pMEVHA-Mst2-Wt & pMEVHA-Mst2-K56R (Kinase-deficient mutant)

20mg

$865.00

PAB1011

Anti-Mst2 Antibody

Purified rabbit polyclonal antibody (0.4 ml at 0.25 mg/ml)  ELISA: 1:1000; Western: 1:100-500; Immunohistochemistry: 1:10-50

100mg

$258.50

H1014

pMEVHA-Lats2 (wts)

Expression-Ready Clones of mouse large tumor suppressor 2 (Lats2) (NM_015771)

20mg

$565.00

PAB1012

Anti-Lats2 Antibody

Purified rabbit polyclonal antibody (0.4 ml at 0.25 mg/ml) ELISA: 1:1000; Western: 1:100-500; Immunohistochemistry: 1:10-50

100mg

$258.50

H1016

pMEVHA-YAP

Expression-Ready Clones of human yes-associated protein 2, (AY316529)

H1016a: pMEVHA-YAP2-WT

H1016b: pMEVHA-YAP2-5SA (phosphorylation sites mutated)

H1016c: pMEVHA-YAP-5SAdC (phosphorylation-deficient and nuclear localization domain deletion mutant, dominant negative)

20mg

$995.00

H1018

pMEVHA-MOB1

Human Mps One binder kinase activator-like 1B, NM_018221

20mg

$565.00

A1010

Human AKT1 Set

Expression-Ready Clones of human AKT1: A1010a: pMEV2HA-AKT1-WT;

A1010b: pMEV2HA-AKT1-K179A; A1010c: pMEV2HA-AKT1-DD;

A1010d: pMEV2HA-AKT1-AA; A1010e: pMEV2HA-AKT1-KAAA

20mg each

$1480.00

MAB1001

HA Tag Antibody

(Mab)

Purified mouse monoclonal antibody (0.4 ml at 0.25 mg/ml) raised with KLH-conjugated synthetic peptide CYPYDVPDYAYPYDVPDYA. ELISA: 1:5000; Western: 1:1000

100mg

$198.00

PAB1001

HA Tag Antibody

(Pab)

Purified rabbit polyclonal antibody (0.4 ml at 0.25 mg/ml) raised with KLH-conjugated synthetic peptide CYPYDVPDYAYPYDVPDYA ELISA: 1:5000; Western: 1:1000

100mg

$198.00

Selected References:

1.         Zhao B. et al., 2007. Genes Dev., 21: 2747-61

2.         Dong J. et al., 2007, Cell, 130: 1120-1133

3.         Zhang L. et al., Dev Cell, Epub March 2008, doi:10.1016/j.devce.2008.01.006

4.         Wu S. et al., Dev Cell, Epub March 2008, doi:10.1016/j.devce.2008.01.006 Camargo FD, et al., 2007, Current Biology, 17: 2054-2060

 

 

 

 

 

 

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