Dominant negatives, Constitutively active mutants, Wild type and mutant expression clones: IRAK1,IRAK4,BRAF,HRAS,NRAS,KRAS2A,KRAS2B,P53, MEKK1, CRAF1, MEK1 IkB, I kappa B alpha,p38,jnk,jnkk1,jnkk2

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ProtéGeneä Expression Ready Clones

Wild Type, Dominant Negative and Constitutively Active Mutants

ProtéGeneTM clones are fully sequenced and expression-verified. If the gene or a mutant of the gene you are interested in cannot be found on the list, please inquire about our custom cloning and mutagenesis services.

 

All genes are cloned in pMEV-2HA driven by optimized CMV promoter for high-level protein expression in mammalian cells. N-terminal 2xHA expression tag increases efficiency in immunoprecipitation or Western blot analysis with anti-HA antibody. The vector also features kanamycin/neomycin gene for bacterial (with kanamycin) or stable mammalian cell selection (with G418).

 

Plasmids provided are expression-ready and transfection purity. Purchase of additional quantities of purified plasmids can be arranged. Please call for quote.

 

pMEV and pMEV-2HA mammalian expression vectors

 

Human H-RAS

Human N-RAS

Human K-RAS2A

Human K-RAS2B

Human P53

Mouse MEKK1

Human CRAF1

Human MEK1

Human c-Jun(1-79)

Human BRAF

Human IκB alpha

Human JNK1

Human p38

Human p38 Gamma

Human JNKK1

Human JNKK2

Human IKK alpha

Human IKK betta

Human AKT1

 

Mammalian Expression Vectors

 

 

 

 

 

 

 

 

 

 

Cat#

Product Name

Brief Description

Amount

Price (USD)

 

Click on hyperlinked names for product manuals and sequences

 

 

P1000

pMEV

Mammalian expression vector designed for high level expression of proteins in mammalian cells using the strong CMV promoter and optimized 5’-leader sequences.

20mg

$195.00

P1001

pMEV-2HA

pMEV-2HA vectors are designed for high level protein expressin in mammalian cells as HA fusions. The use of 2xHA tags increases the detection sensitivity with anti-HA antibodies.

20mg each

$298.00

P1000a

P36 sequencing primer

N-terminal sequencing primer for pMEV and pMEV-2HA
5'-ccgtcagatcactagaagct-3'

0.5nmole

$95.00

P1000b

P43 sequencing primer

C-terminal sequencing primer for pMEV and pMEV-2HA
5'-cttatcatgtctgctcgaagc-3'

0.5nmole

$95.00

 

 

 

 

 

P1010

Human H-RAS Set

Expression-ready clones of human H-Ras.

Set includes:

pMEVHA-HRAS-WT, wild type H-Ras

pMEVHA-HRAS-S17N, dominant negative H-Ras

pMEVHA-HRAS-G12V, constitutively active H-Ras

20mg each

$995.00

P1010a

pMEVHA-HRAS-WT

Expresses wild type human H-Ras1

20mg

$565.00

P1010b

pMEVHA-HRAS-S17N

Dominant negative H-Ras1 (S17N)

20mg

$565.00

P1010c

pMEVHA-HRAS-G12V

Constitutively active H-Ras1 (G12V)

20mg

$565.00

 

 

 

 

 

P1015

Human N-RAS Set

Expression-ready clones of human N-Ras.

Set includes:

pMEVHA-NRAS-WT, wild type N-Ras

pMEVHA-NRAS-S17N, dominant negative N-Ras

pMEVHA-NRAS-G12V, constitutively active N-Ras

20mg each

$995.00

P1015a

pMEVHA-NRAS-WT

Expresses wild type human N-RAS1

20mg

$565.00

P1015b

pMEVHA-NRAS-S17N

Dominant negative N-RAS1 (S17N)

20mg

$565.00

P1015c

pMEVHA-NRAS-G12V

Constitutively active N-RAS1 (G12V)

20mg

$565.00

 

 

 

 

 

P1020

Human KRAS2A Set

Expression-ready clones of human K-Ras2A.

Set includes:

pMEVHA-KRAS2A-WT, wild type K-Ras2A

pMEVHA-KRAS2A-S17N, dominant negative K-Ras2A

pMEVHA-KRAS2A-G12V, constitutively active K-Ras2A

20mg each

$995.00

P1020a

pMEVHA-KRAS2A-WT

Expresses wild type human K-Ras2A1

20mg

$565.00

P1020b

pMEVHA-KRAS2A-S17N

Dominant negative K-Ras2A1(S17N)

20mg

$565.00

P1020c

pMEVHA-KRAS2A-G12V

Constitutively active K-Ras2A1(G12V)

20mg

$565.00

 

 

 

 

 

P1025

Human KRAS2B Set

Expression-ready clones of human K-Ras2B.

Set includes:

pMEVHA-KRAS2B-WT, wild type human K-Ras2B

pMEVHA-KRAS2B-S17N, dominant negative K-Ras2B

pMEVHA-KRAS2B-G12V, constitutively active K-Ras2B

20mg each

$995.00

P1025a

pMEVHA-KRAS2B-WT

Expresses wild type human K-Ras2B1

20mg

$565.00

P1025b

pMEVHA-KRAS2B-S17N

Dominant negative K-Ras2B1(S17N)

20mg

$565.00

P1025c

pMEVHA-KRAS2B-G12V

Constitutively active K-Ras2B1(G12V)

20mg

$565.00

 

 

 

 

 

P1030

Human MEK1 Set

Expression-ready clones of human MEK1. Set includes:

pMEVHA-MEK1-WT, wild type human MEK1

pMEVHA-MEK1-DN, dominant negative MEK1

pMEVHA-MEK1-CA, constitutively active MEK1

pMEVHA-MEK1-K97R, kinase deficient MEK1 (K97R)

20mg each

$1356.00

P1030a

pMEVHA-MEK1-WT

Expresses wild type human MEK1

20mg

$565.00

P1030b

pMEVHA-MEK1-DN

Dominant negative MEK1 (K97R; S218A; S222A)

20mg

$565.00

P1030c

pMEVHA-MEK1-CA

constitutively active MEK1 (Delta amino acid residues32-51; S218E; S222E)

20mg

$565.00

P1030d

pMEVHA-MEK1-K97R

Expresses kinase deficient MEK1 (K97R)

 

$565.00

 

 

 

 

 

P1035

Human P53 Set

Expression-ready clones of human tumor suppressor p53. Set includes:

pMEVHA-P53-WT, wild type p53

pMEVHA-P53-DN, dominant negative p53

20mg each

$865.00

P1035a

pMEVHA-P53-WT

Expresses wild type human P53

20mg

$565.00

P1035b

pMEVHA-P53-DN

Expresses the dominant negative form of p53(V143A)

20mg

$565.00

 

 

 

 

 

P1045

Mouse MEKK1 Set

Expression-ready clones of mouse MEKK1. Set includes:

pMEVHA-MEKK1-KD (CA)

pMEVHA-MEKK1-K452M

20mg each

$865.00

P1045a

pMEVHA-MEKK1-KD

Expresses the kinase domain of wild type mouse MEKK1, amino acid residues 375-672.

It's constitutively active in mammalian cells.

20mg

$565.00

P1045b

pMEVHA-MEKK1-K452M

Expresses the kinase domain (amino acid residues 375-672) of a mutant mouse MEKK1 (K452M).

It lacks protein kinase activity.

20mg

$565.00

 

 

 

 

 

P1060

Human BRAF Set

Expression-ready clones of human BRAF. Set includes:

pMEVHA-BRAF-WT, pMEVHA-BRAF-K482M

pMEVHA-BRAF-ED, pMEV-BRAF-V599E

20mg each

$1365.00

P1060a

pMEVHA-BRAF-WT

Expresses wild type human BRAF

20mg

$565.00

P1060b

pMEVHA-BRAF-K482M

Expresses the kinase deficient human BRAF

20mg

$565.00

P1060c

pMEVHA-BRAF-ED

Expresses constitutively active BRAF (T598E, S601D)

20mg

$565.00

P1060d

pMEV-BRAF-V599E

Expresses the naturally occurring oncogenic mutant, V599E

20mg

$565.00

 

 

 

 

 

P1040

pMEVHA-CRAF1-KD

Expresses the kinase domain of wild type human

CRAF1, amino acid residues 305-648.

20mg

$680.00

 

 

 

 

 

I1000

Human IκBα Set

Expression-ready clones of Human IκB alpha. Set includes: pMEVHA-IκBα wildtype, pMEVHA-IκBα-S(32,36)A

20mg each

$865.00

I1000a

pMEVHA-IκBα-wt

Expresses the wildtype Human IκB alpha

20mg

$565.00

I1000b

pMEVHA-IκBα-S(32,36)A

Expresses the dominant negative form of human IκB alpha (S32A, S36A).

20mg

$565.00

 

 

 

 

 

P1070

Human p38 Set

Expression-ready clones of human p38 (MAPK14). Set includes:pMEVHA-P38-WT, pMEVHA-P38-K53M, pMEVHA-P38-EE and pMEVHA-P38-AA

20mg each

$1356.00

P1070a

pMEVHA-P38-wt

Expression-ready the wildtype human p38 protein kinase

20mg

$565.00

P1070b

pMEVHA-P38-K53M

Expresses the kinase deficient human p38 protein kinase

20mg

$565.00

P1070c

pMEVHA-P38-EE

Expression-ready clone of human P38 mutant with the activation phosphorylation sites T180 and Y182 replaced with glutamate.

20mg

$565.00

P1070d

pMEVHA-P38-AA

Expression-ready clone of human P38 mutant with the activation phosphorylation sites T180 and Y182 replaced with alanine.

20mg

$565.00

 

 

 

 

 

J1105

Human JNK1 Set

Expression-ready clones of human JNK1 (MAPK8). Set includes: pMEVHA-JNK1-WT, pMEVHA-JNK1-K55M, pMEVHA-JNK1-EE and pMEVHA-JNK1-AA

20mg each

$1356.00

J1105a

pMEVHA-JNK1-wt

Expresses the wildtype human JNK1 protein kinase

20mg

$565.00

J1105b

pMEVHA-JNK1-K55M

Expresses the kinase deficient human JNK1 protein kinase

20mg

$565.00

J1105c

pMEVHA-JNK1-EE

Expression-ready clone of human JNK1 mutant with the activation phosphorylation sites T183 and Y185 replaced with glutamate

20mg

$565.00

J1105d

pMEVHA-JNK1-AA

Expression-ready clone of human JNK1 mutant with the activation phosphorylation sites T183 and Y185 replaced with alanine

20mg

$565.00

 

 

 

 

 

P1072

Human P38G Set

Expression-ready clones of human P38 gamma (MAPK12). Set includes: pMEVHA-P38G-WT, pMEVHA-P38G-K56M, pMEVHA-P38G-EE and pMEVHA-P38G-AA

20mg each

$1356.00

P1072a

pMEVHA-P38G-WT

Expression-ready clone of human P38G (MAPK12) wild type

20mg

$565.00

P1072b

pMEVHA-P38G-K56M

Expression-ready clone of a kinase-deficient mutant of human P38G (MAPK12)

20mg

$565.00

P1072c

pMEVHA-P38G-EE

Expression-ready clone of human P38G (MAPK12) mutant with the activation phosphorylation sites T183 and Y185 replaced with glutamate

20mg

$565.00

P1072d

pMEVHA-P38G-AA

Expression-ready clone of human P38G (MAPK12) mutant with the activation phosphorylation sites T183 and Y185 replaced with alanine

20mg

$565.00

 

 

 

 

 

J1106

Human JNKK1 Set

Expression-ready clones of human JNKK1 (MAP2K4). Set includes: pMEVHA-JNKK1-WT, pMEVHA-JNKK1-K131M, pMEVHA-JNKK1-EE and pMEVHA-JNKK1-AA

20mg each

$1356.00

J1106a

pMEVHA-JNKK1-WT

Expression-ready clone of human JNKK1 (MAP2K4) wild type

20mg

$565.00

J1106b

pMEVHA-JNKK1-K131M

Expression-ready clone of a kinase-deficient mutant of human JNKK1 (K131M)

20mg

$565.00

J1106c

pMEVHA-JNKK1-EE

Expression-ready clone of human JNKK1 mutant with the activation phosphorylation sites S257 and T261 replaced with glutamate

20mg

$565.00

J1106d

pMEVHA-JNKK1-AA

Expression-ready clone of human JNKK1 mutant with the activation phosphorylation sites S257 and T261 replaced with alanine

20mg

$565.00

 

 

 

 

 

J1108

Human JNKK2 Set

Expression-ready clones of human JNKK2 (MAP2K7). Set includes: pMEVHA-JNKK2-WT, pMEVHA-JNKK2-K149M, pMEVHA-JNKK2-EE and pMEVHA-JNKK2-AA

20mg each

$1356.00

J1108a

pMEVHA-JNKK2-WT

Expression-ready clone of human JNKK2 (MAP2K7) wild type

20mg

$565.00

J1108b

pMEVHA-JNKK2-K149M

Expression-ready clone of a kinase-deficient mutant of human JNKK2 (K149M)

20mg

$565.00

J1108c

pMEVHA-JNKK2-EE

Expression-ready clone of a human JNKK2 mutant with the activation phosphorylation sites S271 and T275 replaced with glutamate

20mg

$565.00

J1108d

pMEVHA-JNKK2-AA

Expression-ready clone of a human JNKK2 mutant with the activation phosphorylation sites S271 and T275 replaced with alanine

20mg

$565.00

 

 

 

 

 

J1100S

J1100L

GST-cJun

Human c-Jun purified as GST fusion to >90%. Optimal phoshporylation substrate of JNK.

100mg

1.0mg

$249.00

$1999.00

I1009S
I1009L

GST-IKBa

Recombinant murine inhibitor of NFkB alpha (IkBa), amino acid residues 1-93

100mg
1.0mg

$249.00
$1999.00

 

 

 

 

 

A1010

Human AKT1 Set

Expression-ready clones of human AKT1. Set includes:pMEVHA-AKT1-WT, pMEVHA-AKT1-K179A, pMEVHA-AKT1-DD, pMEVHA-AKT1-AA and pMEVHA-AKT1-KAAA

20mg each

$1356.00

A1010a

pMEVHA-AKT1-WT

Expression-ready clone of wild type human AKT1 gene.

20mg

$565.00

A1010b

pMEVHA-AKT1-K179A

See product manual for mutation sites

20mg

$565.00

A1010c

pMEVHA-AKT1-DD

See product manual for mutation sites

20mg

$565.00

A1010d

pMEVHA-AKT1-AA

See product manual for mutation sites

20mg

$565.00

A1010e

pMEVHA-AKT1-KAAA

See product manual for mutation sites

20mg

$565.00

 

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