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ProtéGeneä
Expression Ready Clones |
Wild Type, Dominant Negative and Constitutively Active Mutants |
ProtéGeneTM clones are fully sequenced and expression-verified.
If the gene or a mutant of the gene you are interested in cannot be found on
the list, please inquire about our custom cloning and mutagenesis services. All genes are cloned in
pMEV-2HA driven by optimized CMV promoter for high-level protein expression
in mammalian cells. N-terminal 2xHA expression tag increases efficiency in immunoprecipitation or Western blot analysis with anti-HA
antibody. The vector also features kanamycin/neomycin
gene for bacterial (with kanamycin) or stable mammalian
cell selection (with G418). Plasmids provided are
expression-ready and transfection purity. Purchase of additional quantities
of purified plasmids can be arranged. Please call for quote. |
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Human IKK alpha |
Human IKK betta |
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Cat# |
Product Name |
Brief Description |
Amount |
Price (USD) |
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Click on
hyperlinked names for product manuals and sequences |
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P1000 |
Mammalian expression vector designed for high
level expression of proteins in mammalian cells using the strong CMV promoter
and optimized 5’-leader sequences. |
20mg |
$195.00 |
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pMEV-2HA
vectors are designed for high level protein expressin
in mammalian cells as HA fusions. The use of 2xHA tags increases the
detection sensitivity with anti-HA antibodies. |
20mg each |
$298.00 |
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P1000a |
P36 sequencing primer |
N-terminal sequencing primer for pMEV and pMEV-2HA |
0.5nmole |
$95.00 |
P1000b |
P43 sequencing primer |
C-terminal sequencing primer for pMEV and pMEV-2HA |
0.5nmole |
$95.00 |
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Expression-ready clones of human H-Ras. Set includes: pMEVHA-HRAS-WT,
wild type H-Ras pMEVHA-HRAS-S17N, dominant negative H-Ras pMEVHA-HRAS-G12V, constitutively active H-Ras |
20mg each |
$995.00 |
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P1010a |
pMEVHA-HRAS-WT |
Expresses wild type human H-Ras1 |
20mg |
$565.00 |
P1010b |
pMEVHA-HRAS-S17N |
Dominant negative H-Ras1 (S17N) |
20mg |
$565.00 |
P1010c |
pMEVHA-HRAS-G12V |
Constitutively active H-Ras1 (G12V) |
20mg |
$565.00 |
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Expression-ready clones of human N-Ras. Set includes: pMEVHA-NRAS-WT,
wild type N-Ras pMEVHA-NRAS-S17N, dominant negative N-Ras pMEVHA-NRAS-G12V, constitutively active N-Ras |
20mg each |
$995.00 |
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P1015a |
pMEVHA-NRAS-WT |
Expresses wild type human N-RAS1 |
20mg |
$565.00 |
P1015b |
pMEVHA-NRAS-S17N |
Dominant negative N-RAS1 (S17N) |
20mg |
$565.00 |
P1015c |
pMEVHA-NRAS-G12V |
Constitutively active N-RAS1 (G12V) |
20mg |
$565.00 |
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Expression-ready clones of human K-Ras2A. Set includes: pMEVHA-KRAS2A-WT, wild type K-Ras2A pMEVHA-KRAS2A-S17N, dominant negative K-Ras2A pMEVHA-KRAS2A-G12V, constitutively active K-Ras2A |
20mg each |
$995.00 |
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P1020a |
pMEVHA-KRAS2A-WT |
Expresses wild type human K-Ras2A1 |
20mg |
$565.00 |
P1020b |
pMEVHA-KRAS2A-S17N |
Dominant negative K-Ras2A1(S17N) |
20mg |
$565.00 |
P1020c |
pMEVHA-KRAS2A-G12V |
Constitutively active K-Ras2A1(G12V) |
20mg |
$565.00 |
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Expression-ready clones of human K-Ras2B. Set includes: pMEVHA-KRAS2B-WT, wild type human K-Ras2B pMEVHA-KRAS2B-S17N, dominant negative K-Ras2B pMEVHA-KRAS2B-G12V, constitutively active K-Ras2B |
20mg each |
$995.00 |
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P1025a |
pMEVHA-KRAS2B-WT |
Expresses wild type human K-Ras2B1 |
20mg |
$565.00 |
P1025b |
pMEVHA-KRAS2B-S17N |
Dominant negative K-Ras2B1(S17N) |
20mg |
$565.00 |
P1025c |
pMEVHA-KRAS2B-G12V |
Constitutively active K-Ras2B1(G12V) |
20mg |
$565.00 |
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Expression-ready clones of human MEK1. Set
includes: pMEVHA-MEK1-WT, wild type human MEK1 pMEVHA-MEK1-DN, dominant negative MEK1 pMEVHA-MEK1-CA, constitutively active MEK1 pMEVHA-MEK1-K97R, kinase
deficient MEK1 (K97R) |
20mg each |
$1356.00 |
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P1030a |
pMEVHA-MEK1-WT |
Expresses wild type human MEK1 |
20mg |
$565.00 |
P1030b |
pMEVHA-MEK1-DN |
Dominant negative MEK1
(K97R; S218A; S222A) |
20mg |
$565.00 |
P1030c |
pMEVHA-MEK1-CA |
constitutively active MEK1 (Delta amino acid
residues32-51; S218E; S222E) |
20mg |
$565.00 |
P1030d |
pMEVHA-MEK1-K97R |
Expresses kinase deficient
MEK1 (K97R) |
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$565.00 |
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Expression-ready clones of human tumor suppressor
p53. Set includes: pMEVHA-P53-WT, wild type p53 pMEVHA-P53-DN, dominant negative p53 |
20mg each |
$865.00 |
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P1035a |
pMEVHA-P53-WT |
Expresses wild type human P53 |
20mg |
$565.00 |
P1035b |
pMEVHA-P53-DN |
Expresses the dominant negative form of
p53(V143A) |
20mg |
$565.00 |
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Expression-ready clones of mouse MEKK1. Set
includes: pMEVHA-MEKK1-KD (CA) pMEVHA-MEKK1-K452M |
20mg each |
$865.00 |
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P1045a |
pMEVHA-MEKK1-KD |
Expresses the kinase domain
of wild type mouse MEKK1, amino acid residues 375-672. It's constitutively active in mammalian cells. |
20mg |
$565.00 |
P1045b |
pMEVHA-MEKK1-K452M |
Expresses the kinase
domain (amino acid residues 375-672) of a mutant mouse MEKK1 (K452M). It lacks protein kinase
activity. |
20mg |
$565.00 |
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Expression-ready clones of human BRAF. Set
includes: pMEVHA-BRAF-WT,
pMEVHA-BRAF-K482M pMEVHA-BRAF-ED,
pMEV-BRAF-V599E |
20mg each |
$1365.00 |
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P1060a |
pMEVHA-BRAF-WT |
Expresses wild type human BRAF |
20mg |
$565.00 |
P1060b |
pMEVHA-BRAF-K482M |
Expresses the kinase
deficient human BRAF |
20mg |
$565.00 |
P1060c |
pMEVHA-BRAF-ED |
Expresses constitutively active BRAF (T598E,
S601D) |
20mg |
$565.00 |
P1060d |
pMEV-BRAF-V599E |
Expresses the naturally occurring oncogenic mutant, V599E |
20mg |
$565.00 |
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pMEVHA-CRAF1-KD |
Expresses the kinase domain
of wild type human CRAF1, amino acid residues 305-648. |
20mg |
$680.00 |
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Expression-ready clones of Human IκB alpha. Set includes: pMEVHA-IκBα
wildtype, pMEVHA-IκBα-S(32,36)A |
20mg each |
$865.00 |
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I1000a |
pMEVHA-IκBα-wt |
Expresses the wildtype
Human IκB alpha |
20mg |
$565.00 |
pMEVHA-IκBα-S(32,36)A |
Expresses the dominant negative form of human IκB alpha (S32A, S36A). |
20mg |
$565.00 |
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P1070 |
Expression-ready clones of human p38 (MAPK14). Set
includes:pMEVHA-P38-WT, pMEVHA-P38-K53M, pMEVHA-P38-EE and pMEVHA-P38-AA |
20mg each |
$1356.00 |
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P1070a |
pMEVHA-P38-wt |
Expression-ready the wildtype
human p38 protein kinase |
20mg |
$565.00 |
pMEVHA-P38-K53M |
Expresses the kinase deficient
human p38 protein kinase |
20mg |
$565.00 |
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P1070c |
pMEVHA-P38-EE |
Expression-ready clone of human P38 mutant with
the activation phosphorylation sites T180 and Y182
replaced with glutamate. |
20mg |
$565.00 |
P1070d |
pMEVHA-P38-AA |
Expression-ready clone of human P38 mutant with
the activation phosphorylation sites T180 and Y182
replaced with alanine. |
20mg |
$565.00 |
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J1105 |
Expression-ready clones of human JNK1 (MAPK8).
Set includes: pMEVHA-JNK1-WT, pMEVHA-JNK1-K55M, pMEVHA-JNK1-EE and
pMEVHA-JNK1-AA |
20mg each |
$1356.00 |
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J1105a |
pMEVHA-JNK1-wt |
Expresses the wildtype
human JNK1 protein kinase |
20mg |
$565.00 |
J1105b |
pMEVHA-JNK1-K55M |
Expresses the kinase
deficient human JNK1 protein kinase |
20mg |
$565.00 |
J1105c |
pMEVHA-JNK1-EE |
Expression-ready clone of human JNK1 mutant with
the activation phosphorylation sites T183 and Y185
replaced with glutamate |
20mg |
$565.00 |
J1105d |
pMEVHA-JNK1-AA |
Expression-ready clone of human JNK1 mutant with
the activation phosphorylation sites T183 and Y185
replaced with alanine |
20mg |
$565.00 |
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Expression-ready clones of human P38 gamma
(MAPK12). Set includes: pMEVHA-P38G-WT, pMEVHA-P38G-K56M, pMEVHA-P38G-EE and
pMEVHA-P38G-AA |
20mg each |
$1356.00 |
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P1072a |
pMEVHA-P38G-WT |
Expression-ready clone of human P38G (MAPK12)
wild type |
20mg |
$565.00 |
P1072b |
pMEVHA-P38G-K56M |
Expression-ready clone of a kinase-deficient
mutant of human P38G (MAPK12) |
20mg |
$565.00 |
P1072c |
pMEVHA-P38G-EE |
Expression-ready clone of human P38G (MAPK12)
mutant with the activation phosphorylation sites
T183 and Y185 replaced with glutamate |
20mg |
$565.00 |
P1072d |
pMEVHA-P38G-AA |
Expression-ready clone of human P38G (MAPK12)
mutant with the activation phosphorylation sites
T183 and Y185 replaced with alanine |
20mg |
$565.00 |
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Expression-ready clones of human JNKK1 (MAP2K4). Set
includes: pMEVHA-JNKK1-WT, pMEVHA-JNKK1-K131M, pMEVHA-JNKK1-EE and
pMEVHA-JNKK1-AA |
20mg each |
$1356.00 |
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J1106a |
pMEVHA-JNKK1-WT |
Expression-ready clone of human JNKK1 (MAP2K4)
wild type |
20mg |
$565.00 |
J1106b |
pMEVHA-JNKK1-K131M |
Expression-ready clone of a kinase-deficient
mutant of human JNKK1 (K131M) |
20mg |
$565.00 |
J1106c |
pMEVHA-JNKK1-EE |
Expression-ready clone of human JNKK1 mutant with
the activation phosphorylation sites S257 and T261
replaced with glutamate |
20mg |
$565.00 |
J1106d |
pMEVHA-JNKK1-AA |
Expression-ready clone of human JNKK1 mutant with
the activation phosphorylation sites S257 and T261
replaced with alanine |
20mg |
$565.00 |
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Expression-ready clones of human JNKK2 (MAP2K7).
Set includes: pMEVHA-JNKK2-WT, pMEVHA-JNKK2-K149M, pMEVHA-JNKK2-EE and
pMEVHA-JNKK2-AA |
20mg each |
$1356.00 |
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J1108a |
pMEVHA-JNKK2-WT |
Expression-ready clone of human JNKK2 (MAP2K7)
wild type |
20mg |
$565.00 |
J1108b |
pMEVHA-JNKK2-K149M |
Expression-ready clone of a kinase-deficient
mutant of human JNKK2 (K149M) |
20mg |
$565.00 |
J1108c |
pMEVHA-JNKK2-EE |
Expression-ready clone of a human JNKK2 mutant
with the activation phosphorylation sites S271 and
T275 replaced with glutamate |
20mg |
$565.00 |
J1108d |
pMEVHA-JNKK2-AA |
Expression-ready clone of a human JNKK2 mutant
with the activation phosphorylation sites S271 and
T275 replaced with alanine |
20mg |
$565.00 |
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J1100L |
Human c-Jun purified as GST fusion to >90%.
Optimal phoshporylation substrate of JNK. |
100mg 1.0mg |
$249.00 $1999.00 |
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I1009S |
Recombinant murine
inhibitor of NFkB alpha (IkBa), amino acid residues
1-93 |
100mg |
$249.00 |
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Expression-ready clones of human AKT1. Set
includes:pMEVHA-AKT1-WT, pMEVHA-AKT1-K179A, pMEVHA-AKT1-DD, pMEVHA-AKT1-AA
and pMEVHA-AKT1-KAAA |
20mg each |
$1356.00 |
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A1010a |
pMEVHA-AKT1-WT |
Expression-ready clone of wild type human AKT1
gene. |
20mg |
$565.00 |
A1010b |
pMEVHA-AKT1-K179A |
See product manual for mutation sites |
20mg |
$565.00 |
A1010c |
pMEVHA-AKT1-DD |
See product manual for mutation sites |
20mg |
$565.00 |
A1010d |
pMEVHA-AKT1-AA |
See product manual for mutation sites |
20mg |
$565.00 |
A1010e |
pMEVHA-AKT1-KAAA |
See product manual for mutation sites |
20mg |
$565.00 |
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